Quantitation of Gene Expression Using Capillary Electrophoresis with Laser-induced Fluorescence (CE-LIF)


Reference #:  00505

Invention Description:

The subject invention, capillary electrophoresis with laser-induced fluorescence (CE-LIF), is an attractive alternative for the quantitation of gene expression via fast, sensitive and non-radioactive means.

Potential Applications:

This system could displace current techniquest used to measure genetic expression, including gene-arrays, northern blotting, and real-time polymerase chain reaction (rt-PCR). The potential for using CE-LIF for the direct detection of gene expression extends beyond the basic science applications into the clinical setting enabling disease detection and diagnosis on a rapid and quantitative scale. Another potential application is the detection of bacteria and other infections agents.

Advantages and Benefits:

Capillary electrophoresis with laser induced fluorescence (CE-LIF) can reduce protocol time, eliminate the need for radioactivity and provide superior sensitivity and dynamic range for quantification of RNA. In addition, CE-LIF can be used to directly determine the amount of an RNA species present, something which is difficult and not normally accomplished using current methods.



Quantification of gene expression provides valuable information regarding the response of cells or tissue to stimuli and is often accomplished by monitoring the level of messenger RNA (mRNA) being transcribed for a particular protein. While numerous methods are commonly used to monitor gene expression, including genetic arrays and micro-arrays, northern blotting, rt-PCR with RNase protection, each has its own drawbacks and limitations.




Patent Information:
Title App Type Country Serial No. Patent No. File Date Issued Date Expire Date Patent Status
Direct Quantitation of Gene Expression Using Capillary Electrophoresis with Laser-induced Fluorescence Utility United States 11/455,275 7,939,252 6/16/2006 5/10/2011 10/9/2028 Issued
For Information, Contact:
Technology Commercialization
University of South Carolina
Edie Goldsmith
Jack Goldsmith
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