Detecting Protein Arginine Deiminase (PAD) Activity in Human Tissues and SERA


Reference #: 00844


The abundance of citrullinated proteins in human tissue has become a hallmark of many diseases, including cancer, rheumatoid arthritis, colitis, and multiple sclerosis. Proteins are citrullinated by a class of enzymes termed Protein Arginine Deiminases (PADs), which convert peptidyl-arginine to peptidyl-citrulline through an enzymatic reaction called deimination. Developing PAD inhibitors has become a focus of much research due to the apparent roles of PADs in the aforementioned diseases.

Invention Description:

Dr. Paul Thompson and his research team have developed a novel, easy, straightforward method of measuring PAD activity in mammalian tissue using a solution based assay suitable for a 96-well microplate. The method was developed using mouse tissue and sera, but can readily be adapted to human tissue and sera.

Potential Applications:

The ability to rapidly screen for dysregulated PAD activity in human tissues and sera will provide for early detection and diagnosis of PAD-related diseases.

Advantages and Benefits:

The developed method is simple and straightforward and can be adapted for human tissue and serum with little difficulty. The invention therefore creates a remarkable tool for detecting disease incidence and progression, as well as disease severity.

Patent Information:
Title App Type Country Serial No. Patent No. File Date Issued Date Expire Date Patent Status
Detecting Protein Arginine Deiminase (PAD) actvity in human tissue and sera Utility United States 13/476,379 5/21/2012      
For Information, Contact:
Technology Commercialization
University of South Carolina
Paul Thompson
Lorne Hofseth
Bryan Knuckley
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