PCR Primers for Improved Detection of Vibrio Parahaemolyticus Thermostable Direct Hemolysin (TDH) and TDH-related Hemolysin (TRH) Genes

Description:

Reference# 00977

Invention Description:

The subject invention involves two new sets of PCR primers for the detection of the thermostable direct hemolysin (tdh) and TDH-related hemolysin (trh) genes. Analysis using these new primers detected 28 tdh positive and 5 trh positive strains in a collection of 68 environmental Vibrio isolates. The identities of all PCR products were confirmed by DNA sequence analysis. Screening the same set of isolates with previously described sets of primers (Bej, A.K., D.P. Patterson, C.W. Basher, M.C.L Vickery, D.D Jones and C.A Kaysner. 1999. J. Microbiol. Methods 36:215-225) yielded only 8 tdh positive and 2 trh positive strains.

Background:

The genes encoding the thermostable direct hemolysin (tdh) and the TDH-related hemolysin (trh) are routinely used for detection of pathogenic strains of Vibrio parahaemolyticus in environmental and seafood samples. This organism is the most common cause of seafood associated gastroenteritis and both frequency of its detection and its geographic range are expanding. Commonly used PCR primers for amplification of these genes for screening purposes have been prone to false negative reactions (i.e., no PCR product from samples in which pathogenic V. parahaemolyticus strains are present).

Advantages and Benefits:

  • Facilitate the detection of potentially pathogenic strains of V. parahaemolyticus
  • Improve the sensitivity of detection kits and help to eliminate false negative reactions

Potential Applications:

This invention will be used in the environmental, public health, and food industries to improve detection of pathogenic strains of Vibrio parahaemolyticus.

Patent Information:
Title App Type Country Serial No. Patent No. File Date Issued Date Expire Date Patent Status
PCR Primers for improved detection of Vibrio parahaemolyticus thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) genes PCT PCT PCT/US12/53824   9/6/2012     Expired
For Information, Contact:
Technology Commercialization
University of South Carolina
technology@sc.edu
Inventors:
Charles Lovell
Casandra Gutierrez West
Keywords:
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