Glycomics Tools and Diagnostics: Novel Peptide-Based Borono-Lectin (PBLs) Sugar Sensors

Description:
 

Reference #:  00586

Invention Description:

The present invention is a novel method for design, synthesis, and analysis of peptide-based borono-lectin (PBL) sensors as diagnostic biosensors. Specifically to detect carbohydrates and glycoproteins present in many diseases, such as cancers, inflammation and diabetes. The presented PBL sensors have the potential disrupt current cancer diagnostics because of their stability, biocompatibility, ease of synthesis, and ease of use that will identify the aberrant glycosylation patterns correlated with tumor genesis and metastasis.

 

The technology includes the design and synthesis of a PBL library, the demonstration that selective and cross-reactive PBLs can be identified from this library, and experiments that illustrate the utility of these compounds as disease diagnostics, specifically for cancer.

 

Problem Solved:

While, significant efforts have been made towards the development of sensors or sensing assays for carbohydrates and glycoproteins, several problems have arisen out of the existing technologies.  Most boronic acid-based sensors are poorly water-soluble as their hydrophobic compounds render them ineffective in the aqueous testing environment essential to carbohydrate recognition.  Scaffold synthesis is often difficult, and most sensors consist of toxic or carcinogenic elements.

 

Advantages and Benefits:

The present PBL Sensors introduce unprecedented stability, biocompatibility, ease of synthesis, and ease of use.  These sensors are able to overcome the limitations of boronic acid based sensors and accurately identify the aberrant glycosylation patterns correlated with tumor genesis and metastasis.

 

Potential Applications:

Boronic acids have shown great utility in sensing simple sugars and complex glycoproteins; a differential display of phenylboronic acid (PBA) moieties on a peptide backbone would result in biocompatible, water soluble, cancer diagnostic.  The PBL sensors described in this technology would overcome the limitations of previously described boronic acid based sensors.

 

Development/Demonstration /Validation:  

The synthetic methodology on generating PBL sensors has been established, as has the synthetic protocol to prepare the conjugates of related compounds and glycoproteins. Studies show that selective and cross-reactive PBL sensors can be identified from the complied PBL library, and experiments have demonstrated the utility of these compounds as disease diagnostics.

Patent Information:
For Information, Contact:
Technology Commercialization
University of South Carolina
technology@sc.edu
Inventors:
John Lavigne
Paul Thompson
Keywords:
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